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- Debashish Bhattacherjee
- M. R. Dinesh
- Donald Sangma
- C. Kanupriya
- D. Manmohan Kumar
- P. Nischita
- M. Gayathri
- P. Sampath Kumar
- P. Kavitha
- K. S. Shivashankara
- T. K. Roy
- K. C. Pavithra
- V. K. Rao
- A. T. Sadashiva
- G. J. Sathish
- V. Swarupa
- A. Rekha
- T. S. Aghora
- N. Mohan
- A. H. Naveen
- M. Krishnareddy
- N. Chandrashekar
- R. H. Laxman
- P. Shilpa
- N. Sunil Kumar
- B. Padmakar
- Harish
- V. P. Singh
- N. Divyashree
- N. P. Yashaswini
- M. Sankaran
- N. Chaitra
- Shilpa Pandurangaiah
- K. S. Shivashankar
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- Sunil Gowda
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Journals
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Ravishankar, K. V.
- What Do MBA Students Think of Teacher Evaluations?
Abstract Views :177 |
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Authors
Affiliations
1 Indian Institute of Management, Calcutta, IN
1 Indian Institute of Management, Calcutta, IN
Source
Indian Journal of Industrial Relations: Economics & Social Dev., Vol 51, No 4 (2016), Pagination: 646-659Abstract
Student evaluations of teaching effectiveness (SETE) have been extensively studied in the literature with the central debates revolving around the issues of validity and reliability of the instruments used. Scant attention however has been paid in this literature to student perceptions and views of SETEs and their usefulness. This paper, presents the preliminary results from a survey of MBA students from two Indian business schools where (anonymous) student evaluations of teachers are mandatory after course completion, and where they are also used as one of the key determinants for faculty promotion and tenure decisions. Most students felt that since these SETEs are not taken very seriously by them their reliability are questionable.- Mango Breeding in India - Past and Future
Abstract Views :332 |
PDF Views:148
Authors
Affiliations
1 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru – 560 089, IN
1 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru – 560 089, IN
Source
Journal of Horticultural Sciences, Vol 11, No 1 (2016), Pagination: 1-12Abstract
The mango (Mangifera indica L.) is one of the most important tropical fruits of India in which improvement has been attempted since the early 20th Century. The species, M. indica, having originated in India, has a large diversity within the country. Extensive surveys have located several wild species of importance, many of them figuring in the IUCN Red List. Conservation and evaluation of these species, as well as the large seedling diversity, needs attention as these could be a source for important traits. Strategies of in situ, ex situ and 'onfarm' conservation should from a priority at this juncture. Hybridization has resulted in several hybrids. Widening of genetic base in polyembryonic varieties and identification of zygotic embryos through markers is the need of the hour for utilization in breeding programmes. Although several of these have not become popular, they can be very well used as pre-breeding lines. Use of molecular markers for selection will greatly reduce time taken for developing improved varieties. Strategies other than hybridization, viz., selection among open-pollinated progenies, should be adopted for identifying better recombinants, as, a large number of progenies are available in this method.Keywords
Mango, Mangifera india L., Breeding, Polyembryonic, Monoembryonic.References
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- Dinesh, M.R., Rajan, S., Sanjay Kumar Singh, Singh, I.P., Ravishankar, K.V., Reddy, B.M.C., Parthasarathy. V.A., Bhuwon Sthapit., Ramanatha Rao, V. and Sandya, B.S. 2015. Heirloom/Seedling Mango Varieties of India – Potentialities and Future. Indian J. Pl. Genet. Resour., 28:17-30
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- Ravishankar., K.V., Dinesh, M.R., Nischita, P. and Sandya, B.S. 2015. Development and characterization of microsatellite markers in mango (Mangifera indica L.) using next-generation sequencing technology and their transferability across species. Mol. Breed., 35:93
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- Sane, A., Dinesh, M.R., Ravishankar, K.V., Ravishankar, H. and Vasugi, C. 2015. Implications of polyembryony on the growth performance in mango cultivars. Acta Hort., 1066:47-54
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- Shudo Ayano, Kazuhiko Tarora, Yuko Makishi, Ryotaro Ichi, Ken Takahashi, Masato Matsumura, Sayaka Shimabuku, Noboru Matsuda, Satoshi Nakasone and Naoya Urasaki. 2013. Development of XS markers and their application in breeding for mango, Mangifera indica L. Euphytic, 190:345-355
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- Souza, I.G.B., Valente1, S.E.S., Britto, F.B., de Souza, V.A.B. and Lima, P.S.C. 2011. RAPD analysis of the genetic diversity of mango (Mangifera indica) germplasm in Brazil. Genet. Mol. Res., 10:3080-3089
- Sturrock, T.T. 1968. Genetics of mango polyembryony. Procs. Florida State Horticultural Society, 81:311-314
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- Studies on Genetic Divergence in Pomegranate (Punica granatum L.) Using SRAP Markers
Abstract Views :251 |
PDF Views:137
Authors
C. Kanupriya
1,
D. Manmohan Kumar
1,
P. Nischita
1,
M. Gayathri
1,
K. V. Ravishankar
1,
P. Sampath Kumar
2
Affiliations
1 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, 560089, IN
2 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, 560089, Karnataka, IN
1 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, 560089, IN
2 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, 560089, Karnataka, IN
Source
Journal of Horticultural Sciences, Vol 10, No 2 (2015), Pagination: 125-129Abstract
Pomegranate genotypes have been characterized mainly on the basis of morphological traits; but, these traits are affected to a large extent by environmental and cultivation conditions, resulting in their ambiguous discrimination. Molecular markers are more suited for accurate discrimination of genotypes and cultivars. Sequence-Related Amplified Polymorphism (SRAP) markers were used in the present study to analyze polymorphism among the important pomegranate genotypes grown in India. The total number of bands generated by 30 SRAP primers for 12 genotypes was 1448, with an average of 48.3 bands per primer. Polymorphism varied from 2.7 to 73.9, with an average of 40.95%. Similarity-value based on Jaccard's Coefficient ranged from 0.63 (between cvs. Naina and Amlidana) to 0.95 (between cvs. Kabul Yellow and Jalore Seedless). UPGMA (un-weighted pair group method with arithmetic mean) analysis was performed and a dendrogram was constructed using Jaccard's similarity matrix. The 12 genotypes used grouped into 5 clusters. SRAP markers were found suitable for determining variability among the pomegranate genotypes studied.Keywords
Pomegranate, Molecular Markers, SRAP, Genetic Diversity.References
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- Metabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity
Abstract Views :202 |
PDF Views:131
Authors
P. Kavitha
1,
K. S. Shivashankara
1,
T. K. Roy
1,
K. C. Pavithra
1,
V. K. Rao
1,
A. T. Sadashiva
2,
K. V. Ravishankar
3,
G. J. Sathish
4
Affiliations
1 Division of Plant Physiology and Biochemistry, ICAR-Indian Institute of Horticultural Research, Hesaraghatta lake post, Bengaluru –560 089, IN
2 Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru-560 089, IN
3 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru-560 089, IN
4 Department of Biochemistry, Kuvempu University, Shankaraghatta-577 451, Karnataka, IN
1 Division of Plant Physiology and Biochemistry, ICAR-Indian Institute of Horticultural Research, Hesaraghatta lake post, Bengaluru –560 089, IN
2 Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru-560 089, IN
3 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru-560 089, IN
4 Department of Biochemistry, Kuvempu University, Shankaraghatta-577 451, Karnataka, IN
Source
Journal of Horticultural Sciences, Vol 10, No 1 (2015), Pagination: 30-37Abstract
Vitamins are essential nutrients in food crucial for maintaining good health. Tomato, being a widely consumed vegetable, provides a good quantity of vitamins. Metabolite profiling of vitamins at different stages of fruit maturity in a crop helps identify the right stage for better quality. Based on preliminary screening for quality parameters, tomato lines rich in TSS, antioxidants, lycopene and beta-carotene were selected for the present study. Eight genotypes and a wild species were profiled for 'B' vitamins at three different stages of fruit maturity, viz., green, breaker and ripe stage. A simple and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for simultaneous determination of six 'B' vitamins was developed and validated by us. Among the genotypes studied, IIHR-249-1 recorded higher niacin, pantothenic acid and biotin content. Pyridoxine content was higher in the hybrid, Arka Rakshak. The wild species, LA-1777(Solanum habrochaites) was found to be rich in pantothenic acid, riboflavin and thiamine. Content of most of the vitamins increased with ripening of the fruit. IIHR-249-1 and LA-1777 were found to be rich in 'B' vitamins, earlier reported to be also rich in antioxidants and lycopene. These genotypes can be used for improving the nutritive value of tomato under crop improvement programmes, through conventional breeding or biotechnological approaches.Keywords
Tomato, B Vitamins, LC-MS/MS-MRM, Fruit Ripening, Green Stage, Breaker Stage.References
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- Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides
Abstract Views :168 |
PDF Views:105
Authors
Affiliations
1 Division of Biotechnology, ICAR - Indian Institute of Horticultural Research, Hesaraghatt Lake Post, Bengaluru - 560089, IN
2 Division of Fruit Crops, ICAR - Indian Institute of Horticultural Research, Bengaluru, IN
1 Division of Biotechnology, ICAR - Indian Institute of Horticultural Research, Hesaraghatt Lake Post, Bengaluru - 560089, IN
2 Division of Fruit Crops, ICAR - Indian Institute of Horticultural Research, Bengaluru, IN
Source
Journal of Horticultural Sciences, Vol 9, No 1 (2014), Pagination: 55-60Abstract
Management of the most devasting disease, Fusarium wilt of banana, caused by the fungus Fusarium oxysporum f. sp., cubense, is a challenge to the plant pathologist and the banana grower. Currently, genomics is providing the way for understanding plant defense mechanism, having acquired an important place in crop improvement. To identify the relevant genes and to understand the defense mechanism induced during Fusarium wilt infection, a normalized cDNA library was constructed from infected ischolar_main samples of a tolerant banana genotype, Musa acuminata spp. burmannicoides 'Calcutta-4', by duplex specific nuclease (DSN) based normalization, using the SMART (switching mechanism at 5' end of RNA transcript) full-length cDNA construction method. Sequencing and analysis of 600 clones revealed 392 non-redundant clones. In all, of 88% of the sequences were annotated using Musa genome database, and the remaining 12% were identified as novel loci not annotated. We observed several resistance genes, ROS scavenging genes and genes involved in ubiquitin-proteosome pathway in this study. These genes may have a possible role against Foc infection. These sequences would enrich the EST data developed against specific stress, which is an indispensable tool for predicting functional genes and understanding the defense mechanism.Keywords
Fusarium oxysporum f.sp. cubense, Banana, cDNA Library, Defense Response, Normalization.- Identification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.)
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Authors
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore - 560 089, IN
2 Division of Vegetable Crops, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore - 560 089, IN
3 Division of Plant Pathology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore - 560 089, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore - 560 089, IN
2 Division of Vegetable Crops, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore - 560 089, IN
3 Division of Plant Pathology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore - 560 089, IN
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Journal of Horticultural Sciences, Vol 4, No 2 (2009), Pagination: 167-169Abstract
Mungbean yellow mosaic virus (MYMV) causes yield loss up to 80 % and is becoming problematic in French bean growing areas. Molecular marker linked selection to MYMV resistance is helpful in rapid identification of genotypes carrying resistant genes. Hence, the present study was undertaken to identify the RAPD marker associated with MYMV resistance in French bean (Phaseolus vulgaris L.). Bulk segregant analysis (BSA) was used to identify RAPD marker linked to MYMV resistance. More than 140 random decamers were surveyed for identification of polymorphic markers between the DNA bulks of resistant and susceptible F2 individuals and their contrasting parents. Ninety eight per cent of these primers amplified DNA in both parents and bulks. Twenty two primers produced specific bands for resistant parent which was absent in the susceptible parent. Out of 22 primers, four primers produced specific fragments viz., OPG 13458, OPX 5670, OPW 17380 and OPP 07730, respectively in resistant parent and bulk, which were absent in susceptible parent and bulk. Amplification of individual DNA samples of segregating F2 resistant individuals using putative marker, OPP 07730, a decamer revealed polymorphism in all four resistant and four susceptible F2 segregants, indicating that the marker OPP 07730 was associated with MYMV resistance in IC-525260, a resistant genotype.Keywords
Bulk Segregant Analysis, MYMV, Phaseolus vulgaris L., RAPD Marker.- Physiological and Biochemical Changes during Moisture Stress in Banana
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Authors
Affiliations
1 Department of Biotechnology, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
2 Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru (Karnataka), IN
1 Department of Biotechnology, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
2 Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru (Karnataka), IN
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Asian Journal of Bio Science, Vol 7, No 1 (2012), Pagination: 1-4Abstract
Present investigation was carried out to study the physiological and biochemical changes during moisture stress by identifying two contrasting genotypes from earliar studies i.e. M. acuminata. ssp burmaniceoides commonly recognised as 'Calcutta-4' belonging to AA genomic group and 'Bee hee kela' belonging to BB genomic group were used. The per cent reduction in photosynthetic rate (PN) between control and stressed plants was 45.28 (%) for 'Calcutta-4' and 36.01(%) for 'Bee hee kela', Transpiration rate (E) was 30.24 (%) for 'Calcutta-4' and 22.36 (%) for 'Bee hee kela' and Stomatal conductance (gs) was 60.30 (%) for Calcutta-4 and 56.10 (%) for 'Bee hee kela', indicating BB genotypes are tolerant to water deficit conditions. Leaf water potential (Ø) was higher in 'Bee hee kela' (BB) both in watered (-0.913 MPa) and under stress (-1.518 MPa) situations when compared with 'Calcutta-4' (AA) control (-1.35 Mpa) and stressed (-1.824 MPa) plants. Malondialdehyde content was estimated to be high in 'Calcutta-4' than that of 'Bee hee kela' indicating higher degree of membrane damage in Calcutta-4. The two antioxidant enzymes namely Super oxide dismutase (SOD) and Catalase activities were found to be higher in Bee hee kela stressed samples than 'Calcutta- 4' indicating better oxidative damage withstanding capacity. 'Bee hee kela' (BB) showed higher Ø, gs, and antioxidant enzyme (SOD and Catalase) activities. 'Bee hee kela' genotype is more drought tolerant than 'Calcutta-4'. Identified drought tolerant genotype can be further used as a donor for drought tolerance.Keywords
Banana, Drought, Photosynthesis, Melondialdehyde, Antioxidants.- Molecular Mechanisms Involved in Biosynthesis and Regulation of Carotenoids in Plants
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Authors
Affiliations
1 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Bengaluru 560 089, IN
2 Division of Biotechnology, ICAR- Indian Institute of Horticultural Research, Bengaluru 560 089, IN
3 Division of Plant Physiology and Biochemistry, ICAR- Indian Institute of Horticultural Research, Bengaluru 560 089, IN
1 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Bengaluru 560 089, IN
2 Division of Biotechnology, ICAR- Indian Institute of Horticultural Research, Bengaluru 560 089, IN
3 Division of Plant Physiology and Biochemistry, ICAR- Indian Institute of Horticultural Research, Bengaluru 560 089, IN
Source
Journal of Horticultural Sciences, Vol 11, No 2 (2016), Pagination: 91-103Abstract
Carotenoids are coloured compounds beneficial to plants and humans. Some of the major health benefits carotenoids provide include Vitamin A precursors and, antioxidants besides being involved in several physiological functions. Even though several carotenoids are synthesised by plants, only a few like beta/ alpha carotenes and cryptoxanthin serve as Vitamin A precursors. The rest are useful as antioxidants. To draw maximum benefits from carotenoids, we need to incorporate these in crop improvement programmes for enhancing available Vitamin A precursor carotenoids. Therefore, it is essential to study biosynthesis of carotenoids, their genetics and their control. In this review, we focus on factors regulating carotenoid biosynthesis, metabolism and storage in plastids. Transcriptional and genetic control of carotenoid production in plants is discussed in the review using several mutants too. Further, environmental regulation of carotenoid biosynthesis is also highlighted. Carotenoid-rich fruits and vegetables have greater economic value owing to their health-promoting effects. Besides,carotenoids have several industrial applications. Therefore, knowledge of regulation mechanism in carotenoid production in plants can help develop crop varieties or technologies, thus generating carotene-rich fruits and vegetables.Keywords
Carotenoid Biosynthesis, Regulation, Plastid, Fruit, Transcription Factor.References
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- Marker-Trait Association for Fruit Characters in Mango (Mangifera indica L.) Cultivars
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Authors
Affiliations
1 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research Hessaraghatta Lake Post, Bengaluru 560089, Karnataka, IN
2 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research Hessaraghatta Lake Post, Bengaluru 560089, Karnataka, IN
1 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research Hessaraghatta Lake Post, Bengaluru 560089, Karnataka, IN
2 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research Hessaraghatta Lake Post, Bengaluru 560089, Karnataka, IN
Source
Journal of Horticultural Sciences, Vol 11, No 2 (2016), Pagination: 170-178Abstract
In the present study, putative marker-trait associations were identified within a core collection of mango cultivars by simple-sequence-repeat marker based association study. A panel of 48 mango varieties which represented the core collection of the South-West region of India, were characterized at the molecular level using 31 simple sequence repeat markers. Morphological characterization included important fruit characteristics viz., fruit weight, total soluble solids (TSS), pulp content and acidity. The study on population structure revealed two sub-groups in the core collection. Association analysis, computed by General Linear Model (GLM), using TASSEL resulted in the identification of seven markers being associated with the trait titrable acidity where as one marker each of the traits fruit weight and TSS. These traitspecific markers were highly significant at p<0.05 and explained a good amount of phenotypic variation by exhibiting substantial R2 values ranging from 0.71 to 0.86 for acidity, 0.61 for TSS and 0.59 for fruit weight. This is the first report on marker-trait associations (MTA) in mango.Keywords
Marker-Trait Association, Fruit Characters, Mango, Mangifera indica L.References
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- RAPD Assisted Diversity Analysis of Scented Geranium Lines (DNA Finger Printing)
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Authors
Affiliations
1 College of Horticulture, Mysore (Karnataka), IN
2 College of Horticulture, University of Horticultural Sciences, Bagalkot, Udyanagiri (Karnataka), IN
3 Indian Institute of Horticultural Research, Bengaluru (Karnataka), IN
1 College of Horticulture, Mysore (Karnataka), IN
2 College of Horticulture, University of Horticultural Sciences, Bagalkot, Udyanagiri (Karnataka), IN
3 Indian Institute of Horticultural Research, Bengaluru (Karnataka), IN
Source
International Journal of Plant Sciences, Vol 14, No 1 (2019), Pagination: 44-50Abstract
Scented geranium (Pelargonium spp.: Geraniaceae) is an important, high value aromatic crop of South African origin. Due to high demand and price for the oil, an excellent potential exists for increasing cultivated area in India. An attempt was made here to evaluate and characterize the available accessions of scented geranium based on their molecular (genetic) diversity. The present study was done using 14 accessions of scented geranium for RAPD analysis. Twenty arbitrary 10-mer oligonucleotide primers amplified 220 markerks, out of which 119 were polymorphic. Each primer amplified on an average five polymorphic bands. The total number of bands produced by each primer ranged from 7 to 16 with an average of 11 bands per primeramong which, The primers OPF-2, OPF-4, OPG-11, OPG-18, OPX-9, OPX-11, OPX-13, OPX- 14 and OPX-15 proved much more useful in differentiating the accessions. The different accessions used in the investigation were grounded into two major clusters. Group I comprises of 13 accessions where it has been further subdivided into two subgroups. Subgroup I comprises of PG-1, PG-7, Kolar-C, CIMAP, Hemanti, Kunti, Kodaikanal Bourbon, PG-12, Bipuli and Kolar-N. Among these, PG-12 and Bipuli are closely related at a linkage distance of approximately 15, Kolar-N is related to these two, whereas PG-1 and PG-7 are related by a linkage distance of approximately 24, which are linked to Kolar-C and CIMAP. Kunti and Kodiakanal Bourbon formed a cluster and are linked by a distance of approximately 43. Subgroup II of Group I consists of PG-8, PG-10 and PG-11. Among these, PG-8 and PG-10 originated from a single node with a linkage distance of approximately 37, whereas, PG-11 is related to these two. Kelkar, which stood apart in a separate node in comparison with the remaining 13 acccessions. The linkage distance among the accessions as revealed by distance matrix ranged from 15 to 164. The cluster analysis (Dendrogram) using ward’s method also revealed that, PG-12 and Bipuli are more related to each other and KN appears to be related to these two. PG-1 and PG-7, which were closely related, showed similarly in leaf morphology and growth pattern. Kolar-C and CIMAP are linked to these two. Hemanti’ was linked with PG-1 PG-7, KC and CIMAP. The principal component analysis (PCA) also clearly shows the distinctiveness of the accession Egyptian (Kelkar) from the rest, while the grouping of different accessions is similar to the one, which is obtained in dendrogram.Keywords
Geranium, Accessions, Genetic Diversity, RAPD Analysis.References
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- Morphological, Cytological, Palynological and Molecular Characterization of Certain Mangifera Species
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Authors
Affiliations
1 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Bengaluru 560 089, IN
2 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Bengaluru 560 089, IN
1 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Bengaluru 560 089, IN
2 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Bengaluru 560 089, IN
Source
Current Science, Vol 115, No 7 (2018), Pagination: 1379-1386Abstract
The Mangifera genus has more than 60 species, mostly distributed in tropical Asia. The wild relatives of Mangifera are considered reservoirs of potential genes that can confer tolerance/resistance to biotic and abiotic stresses. The morphological, cytological and molecular characterization of eight species was done to study the diversity and phylogenetic relationship among different Mangifera species. In order to study the evolutionary relationship and polymorphism among the mango species, the ITS1/ITS4 gene and partial chloroplast psbH-trnH genes were sequenced. Phylogenetic analysis of the nuclear and chloroplast marker revealed that the M. indica L. is closely related to M. griffithii and M. camptosperma, which belong to subgenus Mangifera. Results indicate that the taxonomic position of M. andamanica should be reconsidered as this species is very close to Bouea oppositifolia which is evident from both ITS and psbAtrnH rDNA analysis. The morphological traits such as tree, leaf, flowers and fruits and palynological and cytology of the genus mango were used to distinguish the species and its phylogentic status. The morphological traits among various species indicate the high level of variability which were further confirmed with ITS sequences and cpDNA. Phylogenetic analysis illustrates that partial chloroplast psbH-trnH gene gave better polymorphism in mango species than nuclear ITS. The pollen morphology and chromosomal counts were also done in certain Mangifera species to study the phylogenetic relationship.Keywords
Chromosome, ITS, Mango, Pollen Grains, psbA-trnH and Phylogenetic Analysis.References
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- Carotenoid Content in Cherry Tomatoes Correlated to the Color Space Values L*, a*, b*: A Non-destructive Method of Estimation
Abstract Views :526 |
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Authors
Shilpa Pandurangaiah
1,
A. T. Sadashiva
1,
K. S. Shivashankar
1,
D. V. Sudhakar Rao
1,
K. V. Ravishankar
1
Affiliations
1 ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, IN
1 ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, IN
Source
Journal of Horticultural Sciences, Vol 15, No 1 (2020), Pagination: 27-34Abstract
Cherry tomatoes are rich sources of carotenoids. The carotenoids are known to be precursors of vitamin A and also act as an antioxidant. It is important to visually judge the tomato surface color for higher β carotene content since this is the major provitamin A carotenoid. Estimation of carotenoids by HPLC (High Performance Liquid Chromatography) and spectrophotometric methods in tomatoes are very expensive and time consuming. Therefore, colorimeters can be used to describe the color and determine the carotenoid content in a relatively easy and inexpensive manner. The objective of this study was to determine, if the carotenoid content within cherry tomatoes measured by conventional method could correlate with colorimetric CIE (Commission International del’Eclairage) L*, a*, b* color space values. Strong correlations were found between color surface value a* and total carotenoids (0.82) and lycopene content (0.87). We also observed positive correlation for the b* color value with β carotene (0.86). The L* value was negatively correlated (-0.78) with an increase in carotenoids. These close associations between color space values L*, a*, b* and carotenoids will help the breeders to quickly screen large germplasm/ breeding lines in their breeding program for improvement in carotenoid content through this time saving, inexpensive and nondestructive method at fully ripe stage.Keywords
β Carotene, Carotenoid, Lycopene, Tomato.References
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- Varate Giduga (Acc. No. 21067; IC No. 418238) : A unique mango (Mangifera indica L.) variety
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Authors
Affiliations
1 ICAR-Indian Institute of Horticultural Research, Bengaluru - 560 089, IN
1 ICAR-Indian Institute of Horticultural Research, Bengaluru - 560 089, IN
Source
Journal of Horticultural Sciences, Vol 14, No 2 (2019), Pagination: 161-161Abstract
No Abstract.- M. Udayakumar (1946–2021)
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Authors
Affiliations
1 Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 Division of Biotechnology, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
3 I&B Seeds Pvt Ltd, Bengaluru 560 060, IN
1 Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 Division of Biotechnology, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
3 I&B Seeds Pvt Ltd, Bengaluru 560 060, IN